In order to predict thermophilic proteins please use the python version 3.7.6 and follow the following steps:

(1) First download the iThermo software pakage

(2) Access iThermo in window command prompt, to fullfill the requirements of iThermo run "pip install -r requirements.txt"

(3) Paste the protein sequence in FASTA format in insample.txt file available in input directory of sofware pakage

(4) After the pasting sequence run this command as it is "python iThermo.py ./input/insample.txt ./output/result.txt"

(5) Find the result in reslts.txt file which is available in output directory of software pakage

(6) To download the benchmark dataset, training and independent dataset click on Downloads