The web-server ChloPred was developed to identify subchloroplast location of proteins based on the sequence information. The binomial distribution was used to find out optimized tripeptide. The anticipated overall success rates are 88.03% by using jackknife cross-validation. 92.21% envelope proteins, 93.20% thylakoid membrane, 52.63% thylakoid lumen and 85.00% stroma can be correctly identified. All data can be downloaded from the Data window of this web-server.

Caveat

(1) For each submission, the number of protein sequences is limited at 100 or less;

(2) The input sequences must be in FASTA format; i.e., each protein sequence should start with a greater-than symbol (" > ") in the first column. The words right after the " > " symbol in the single initial line are optional and only used for the purpose of identification and description.

(3) If a query sequence contains any illegal character, wrong results will be obtained.