Physicochemical properties GG GA GC GT AG AA AC AT CG CA CC CT TG TA TC TT Base stacking -0.07 -0.65 -2.46 -0.92 0.49 1.02 -0.92 0.57 -0.58 0.57 -0.07 0.49 0.57 1.6 -0.65 1.02 Protein induced deformability 0.36 -0.14 -0.3 -0.83 -0.89 -0.64 -0.83 -1.05 2.23 1.51 0.36 -0.89 1.51 0.42 -0.14 -0.64 B-DNA twist -0.06 1.11 0.79 -0.65 -1.33 0 -0.65 2.09 -1.14 0.6 -0.06 -1.33 0.6 -1.06 1.11 0 Dinucleotide GC Content 1.37 0 1.37 0 0 -1.37 0 -1.37 1.37 0 1.37 0 0 -1.37 0 -1.37 A-philicity -1.19 1.02 0.32 -1.36 -0.8 0.99 -1.36 -0.1 -0.27 1.19 -1.19 -0.8 1.19 0.32 1.02 0.99 Propeller twist 1.4 -0.27 0.47 -0.16 -0.44 -1.89 -0.16 -0.75 0.8 0.98 1.4 -0.44 0.98 0.23 -0.27 -1.89 Duplex stability-free energy -1.23 0.27 -1.23 0.27 0.27 0.83 0.27 1.4 -2.17 -0.11 -1.23 0.27 -0.11 1.4 0.27 0.83 Duplex stability-disrupt energy 1.4 -0.5 1.4 -0.88 -0.5 -0.12 -0.88 -1.39 2.04 -0.12 1.4 -0.5 -0.12 -0.63 -0.5 -0.12 DNA denaturation 0.67 -0.22 2.35 1.11 0.02 -0.84 1.11 -0.58 0.19 -0.91 0.67 0.02 -0.91 -1.6 -0.22 -0.84 Bending stiffness 2.08 -0.12 0.67 -0.12 -0.12 -0.9 -0.12 -1.37 0.67 -0.12 2.08 -0.12 -0.12 -1.37 -0.12 -0.9 Protein DNA twist -0.5 0.83 -0.22 -1.04 -0.88 0.36 -1.04 -1.9 0.75 1.22 -0.5 -0.88 1.22 1.41 0.83 0.36 Stabilising energy of Z-DNA -0.58 0.15 0.59 1.03 0.15 0.52 1.03 1.97 -1.82 -1.38 -0.58 0.15 -1.38 -0.51 0.15 0.52 Aida_BA_transition -0.5 -0.19 1.52 0.26 -0.99 -0.93 0.26 1.03 2.36 0.45 -0.5 -0.99 0.45 -1.11 -0.19 -0.93 Breslauer_dG 1.4 -0.5 1.4 -0.88 -0.5 -0.12 -0.88 -0.63 2.04 -0.12 1.4 -0.5 -0.12 -1.39 -0.5 -0.12 Breslauer_dH 1.31 -1.1 1.35 -0.7 -0.12 0.46 -0.7 0.23 1.71 -1.02 1.31 -0.12 -1.02 -0.93 -1.1 0.46 Breslauer_dS 1.14 -1.25 1.16 -0.56 0.08 0.67 -0.56 0.65 1.36 -1.36 1.14 0.08 -1.36 -0.63 -1.25 0.67 Electron_interaction 1.37 0 1.37 0 0 -1.37 0 -1.37 1.37 0 1.37 0 0 -1.37 0 -1.37 Hartman_trans_free_energy -0.49 -0.41 -1.71 -1.28 0.23 0.59 -1.28 -0.49 0.67 1.1 -0.49 0.23 1.1 2.03 -0.41 0.59 Helix-Coil_transition 1.41 -0.49 1.41 -0.89 -0.49 -0.13 -0.89 -0.62 2.04 -0.13 1.41 -0.49 -0.13 -1.37 -0.49 -0.13 Ivanov_BA_transition -1.19 1.02 0.32 -1.36 -0.8 0.99 -1.36 -0.1 -0.27 1.19 -1.19 -0.8 1.19 0.32 1.02 0.99 Lisser_BZ_transition -0.49 -0.4 -1.71 -1.28 0.23 0.59 -1.28 -0.49 0.66 1.09 -0.49 0.23 1.09 2.04 -0.4 0.59 Polar_interaction 1.37 0 1.37 0 0 -1.37 0 -1.37 1.37 0 1.37 0 0 -1.37 0 -1.37 SantaLucia_dG 0.86 0.17 2 0.1 -0.5 -0.81 0.1 -1.46 1.57 -0.01 0.86 -0.5 -0.01 -1.75 0.17 -0.81 SantaLucia_dH -0.73 -0.03 2.35 0.6 -1.15 0.46 0.6 -0.87 1.65 -0.24 -0.73 -1.15 -0.24 -1.01 -0.03 0.46 SantaLucia_dS -1.28 -0.04 2.1 0.68 -1.14 0.83 0.68 -0.43 1.34 -0.3 -1.28 -1.14 -0.3 -0.51 -0.04 0.83 Sarai_flexibility -0.79 -0.43 0.08 0.85 -0.57 -0.7 0.85 3.16 -0.41 0.03 -0.79 -0.57 0.03 0.39 -0.43 -0.7 Stability 0.5 0.52 2.52 0.97 -0.61 -0.77 0.97 -0.67 -0.76 -0.04 -0.76 0.5 -0.61 -1.49 0.52 -0.77 Stacking_energy 0.07 0.65 2.45 0.91 -0.49 -1.02 0.91 -0.57 0.61 -0.57 0.07 -0.49 -0.57 -1.6 0.65 -1.02 Sugimoto_dG 0.96 -0.24 1.35 -0.24 -0.24 -0.83 -0.24 -1.43 2.35 0.16 0.96 -0.24 0.16 -1.43 -0.24 -0.83 Sugimoto_dH 1.26 0.09 1.04 0.42 -1.14 -0.36 0.42 -1.71 1.77 -0.25 1.26 -1.14 -0.25 -1.14 0.09 -0.36 Sugimoto_dS 1.29 0.19 0.84 0.64 -1.41 -0.17 0.64 -1.68 1.43 -0.37 1.29 -1.41 -0.37 -0.96 0.19 -0.17 Watson-Crick_interaction 1.37 0 1.37 0 0 -1.37 0 -1.37 1.37 0 1.37 0 0 -1.37 0 -1.37 Twist 0.06 -0.08 -0.08 1.5 0.78 0.06 1.5 1.07 -1.66 -1.38 0.06 0.78 -1.38 -1.23 -0.08 0.06 Tilt 1.08 0.5 0.22 0.5 0.36 0.5 0.5 0.22 -1.22 -1.36 1.08 0.36 -1.36 -2.37 0.5 0.5 Roll -0.28 0.09 2.3 1.19 -0.28 0.09 1.19 0.83 -1.38 -1.01 -0.28 -0.28 -1.01 -1.38 0.09 0.09 Shift 0.56 0.13 -0.35 0.13 0.68 1.59 0.13 -1.02 -0.82 -0.86 0.56 0.68 -0.86 -2.24 0.13 1.59 Slide -0.82 -0.39 0.65 1.29 -0.24 0.11 1.29 2.51 -0.29 -0.62 -0.82 -0.24 -0.62 -1.51 -0.39 0.11 Rise 0.24 0.71 1.59 1.04 -0.62 -0.11 1.04 1.17 -1.39 -1.25 0.24 -0.62 -1.25 -1.39 0.71 -0.11 Stacking energy 1.99 0.16 -1.57 -0.22 0.07 -0.41 -0.22 0.93 -1.47 -0.7 1.99 0.07 -0.7 0.35 0.16 -0.41 Bend -0.8 -0.47 0.03 -0.05 -0.66 0.04 -0.05 -0.39 -0.2 0.51 -0.8 -0.66 0.51 3.42 -0.47 0.04 Tip -0.34 0.01 0.62 0.35 -0.24 0.23 0.35 0.28 -0.6 -1.6 -0.34 -0.24 -1.6 2.88 0.01 0.23 Inclination 1.24 -0.37 0 0.12 -1.03 -1.6 -0.12 0 0 1.47 -1.24 1.03 -1.47 0 0.37 1.6 Major Groove Width 1.79 0.38 0.94 -1.04 0 -1.24 -1.04 -0.58 0.82 0.06 1.79 0 0.06 -1.08 0.38 -1.24 Major Groove Depth -1.4 -0.48 -0.75 1.44 0.11 0.58 1.44 0.11 -0.34 -0.75 -1.4 0.11 -0.75 2 -0.48 0.58 Major Groove Size 0 -1.37 -1.37 0 1.37 0 0 1.37 1.37 0 0 1.37 0 -1.37 -1.37 0 Major Groove Distance -0.05 0.02 -1.28 -1.31 -0.13 -0.05 -1.31 -1.35 1.35 1.42 -0.05 -0.13 1.42 1.49 0.02 -0.05 Minor Groove Width -1.02 -0.85 -0.8 1.55 0.01 0.21 1.55 0.23 -0.04 -0.71 -1.02 0.01 -0.71 2.2 -0.85 0.21 Minor Groove Depth -0.06 1.17 -0.16 -2.1 -0.16 0.35 -2.1 -0.88 0.66 0.97 -0.06 -0.16 0.97 0.04 1.17 0.35 Minor Groove Size 0.92 -0.92 -0.41 -0.41 0.92 -0.92 -0.41 -0.41 2.26 0.41 0.92 0.92 0.41 -1.44 -0.92 -0.92 Minor Groove Distance -0.79 -0.54 1.68 1.64 -0.81 -0.55 1.64 1.61 -0.09 -0.3 -0.79 -0.81 -0.3 -0.5 -0.54 -0.55 Persistance Length 2.08 -0.12 0.67 -0.12 -0.12 -0.9 -0.12 -1.37 0.67 -0.12 2.08 -0.12 -0.12 -1.37 -0.12 -0.9 Melting Temperature 0.5 0.52 2.52 0.97 -0.61 -0.77 0.97 -0.67 -0.04 -0.76 0.5 -0.61 -0.76 -1.49 0.52 -0.77 Mobility to bend towards major groove -1.02 0.55 -1.42 -0.63 0.16 2.52 0.16 1.34 -0.63 0.16 -1.22 -0.23 -0.43 0.36 -0.43 0.75 Mobility to bend towards minor groove 1.32 -0.26 0.35 -0.38 -0.62 -1.23 -0.5 -1.47 1.32 0.23 1.56 0.23 1.07 -1.1 0.71 -1.23 Propeller Twist -0.08 -0.73 0.24 1.65 -0.5 -1.35 1.65 -1.24 0.38 1.11 -0.08 -0.5 1.11 0.4 -0.73 -1.35 Clash Strength 0.75 -1.21 -1 -0.18 1.59 -0.53 -0.18 0.64 1.46 -0.35 0.75 1.59 -0.35 -1.24 -1.21 -0.53 Enthalpy -1.26 -0.09 -1.04 -0.42 1.14 0.36 -0.42 1.71 -1.77 0.25 -1.26 1.14 0.25 1.14 -0.09 0.36 Free energy -0.93 0.17 -1.7 -0.24 0.05 1.32 -0.24 1.45 -1.75 -0.01 -0.93 0.05 -0.01 1.25 0.17 1.32 Twist_twist 0.76 0.16 0.16 0.87 0.36 0.56 0.87 0.56 -1.76 -1.97 0.76 0.36 -1.97 -0.45 0.16 0.56 Tilt_tilt 0.81 0.45 0.63 0.81 0.1 0.45 0.81 0.63 -1.49 -1.49 0.81 0.1 -1.49 -2.02 0.45 0.45 Roll_roll 0.38 -0.32 1.32 1.08 0.15 0.38 1.08 1.08 -1.73 -1.02 0.38 0.15 -1.02 -1.96 -0.32 0.38 Twist_tilt -0.2 0.2 -0.2 0.2 -1.4 2.2 0.2 -0.2 0.2 -0.6 -0.2 -1.4 -0.6 -0.6 0.2 2.2 Twist_roll -1.36 1.23 0.19 0.71 -0.32 0.71 0.71 0.71 -1.88 -0.84 -1.36 -0.32 -0.84 0.71 1.23 0.71 Tilt_roll -0.73 -0.22 -0.73 1.3 -1.75 1.3 1.3 0.29 0.29 0.29 -0.73 -1.75 0.29 -0.22 -0.22 1.3 Shift_shift 1.42 -0.7 0.57 -1.04 0.17 1.38 -1.04 -1.6 -1.02 -0.05 1.42 0.17 -0.05 -0.32 -0.7 1.38 Slide_slide 1.3 -0.53 -0.04 0.66 -0.04 0.5 0.66 1.54 -1.81 -1.14 1.3 -0.04 -1.14 -1.19 -0.53 0.5 Rise_rise -0.16 0.39 1.45 1.23 -0.67 0 1.23 1.57 -1.76 -0.73 -0.16 -0.67 -0.73 -1.4 0.39 0 Shift_slide 0.47 0.28 2.03 -0.81 -0.03 1.15 -0.81 -0.45 -0.99 -1.57 0.47 -0.03 -1.57 0.44 0.28 1.15 Shift_rise 1.62 -0.17 0.83 -0.46 -0.71 1.4 -0.46 -0.63 -0.8 -1.02 1.62 -0.71 -1.02 -0.72 -0.17 1.4 Slide_rise -0.5 -0.57 1.6 1.66 -0.31 -0.22 1.66 1.49 -0.08 -1.09 -0.5 -0.31 -1.09 -0.94 -0.57 -0.22 Twist_shift 0.7 -0.27 -0.27 -0.04 -1.44 1.99 -0.04 -0.55 0.66 -0.62 0.7 -1.44 -0.62 -0.47 -0.27 1.99 Twist_slide -0.89 -0.63 -0.45 -0.56 -0.21 -0.8 -0.56 0.63 1.04 2.01 -0.89 -0.21 2.01 0.95 -0.63 -0.8 Twist_rise 0.81 0.21 -1.15 -1.15 -0.47 -1.11 -1.15 1.87 0.38 0.3 0.81 -0.47 0.3 1.7 0.21 -1.11 Tilt_shift -1.69 -0.32 -1.09 0.42 -0.07 0.67 0.42 1.44 -1.23 0.74 -1.69 -0.07 0.74 1.4 -0.32 0.67 Tilt_slide 0.84 0.04 -1.81 -1.23 1.19 -0.66 -1.23 -0.89 1.42 0.04 0.84 1.19 0.04 0.84 0.04 -0.66 Tilt_rise -0.54 -0.53 -0.28 1.03 1.11 -2.02 1.03 0.57 0.56 0.3 -0.54 1.11 0.3 0.46 -0.53 -2.02 Roll_shift -0.66 0.73 -0.48 1.01 -1.6 1.2 1.01 0.55 0.73 -0.48 -0.66 -1.6 -0.48 -1.22 0.73 1.2 Roll_slide 0.25 0.62 1.04 0.55 -1.1 -0.67 0.55 -2.38 -0.61 1.23 0.25 -1.1 1.23 0.19 0.62 -0.67 Roll_rise -0.01 -0.25 1.76 1.51 -0.09 -0.86 1.51 1.28 -0.36 -1.34 -0.01 -0.09 -1.34 -0.61 -0.25 -0.86 Slide stiffness -0.82 -0.39 0.65 1.29 -0.24 0.11 1.29 2.51 -0.29 -0.62 -0.82 -0.24 -0.62 -1.51 -0.39 0.11 Shift stiffness 0.56 0.13 -0.35 0.13 0.68 1.59 0.13 -1.02 -0.82 -0.86 0.56 0.68 -0.86 -2.24 0.13 1.59 Roll stiffness -0.28 0.09 2.3 1.19 -0.28 0.09 1.19 0.83 -1.38 -1.01 -0.28 -0.28 -1.01 -1.38 0.09 0.09 Rise stiffness 0.24 0.71 1.59 1.04 -0.62 -0.11 1.04 1.17 -1.39 -1.25 0.24 -0.62 -1.25 -1.39 0.71 -0.11 Tilt stiffness 1.08 0.5 0.22 0.5 0.36 0.5 0.5 0.22 -1.22 -1.36 1.08 0.36 -1.36 -2.37 0.5 0.5 Twist stiffness 0.06 -0.08 -0.08 1.5 0.78 0.06 1.5 1.07 -1.66 -1.38 0.06 0.78 -1.38 -1.23 -0.08 0.06 Wedge -0.87 0.34 0.23 -1.25 1.52 1.06 -1.25 -0.68 0.87 -0.34 -0.87 1.52 -0.34 -1.33 0.34 1.06 Direction 0.44 1.05 1.62 -1.48 -0.09 -1.59 1.27 -0.11 -0.11 -0.72 -0.66 -0.13 0.51 -0.11 -1.26 1.37 Flexibility_slide -0.15 0.62 -0.95 0.43 -0.09 1.53 0.43 1 -1.04 -1.74 -0.15 -0.09 -1.74 -0.21 0.62 1.53 Flexibility_shift -0.22 -0.25 -1.42 1.2 0.95 -0.28 1.2 -1.7 2.01 -0.53 -0.22 0.95 -0.53 -0.64 -0.25 -0.28 Entropy 1.17 -0.1 -1.32 -0.21 0.56 0.4 -0.21 0.9 -2.87 -0.38 1.17 0.56 -0.38 0.4 -0.1 0.4