The benchmark dataset used in this web-server consists of a positive dataset and a negative dataset. The positive dataset contains 5,000 nucleosome-forming DNA segments, while the negative dataset contains 5,000 nucleosome-inhibiting (or linker) DNA segments derived from experimental data . Each of these segments is 150-bp long. The benchmark dataset can be downloaded.
 Lee, W., Tillo, D., Bray, N., Morse, R.H., Davis, R.W., Hughes, T.R. and Nislow, C. (2007) A high resolution atlas of nucleosome occupancy in yeast. Nat. Genet. 39, 1235-1244.